| 质粒名称: | pDONR223_CHD2_WT |
|---|---|
| 目录编号: | 81768 |
| 存储实验室: | Jesse Boehm, William Hahn, David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pDONR223 |
| 载体出品公司: | Invitrogen |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 4299bp |
| 载体类型: | Gateway Entry vector |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 大观霉素，50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | CHD2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 2790 bp |
| 基因突变: | WT |
| GenBank编号 | JN247409 |
| 启动子: | none |
| 标签/融合蛋白: | CHD2 (a.k.a. EEOC) |
| 克隆方法: | Gateway Cloning |
| 5’测序引物 | M13pUC-fwd |
| 3’测序引物 | M13-Rev |
|
文献引用方法 |
|
| 材料和方法部分: | pDONR223_CHD2_WT was a gift from Jesse Boehm & William Hahn & David Root (Addgene plasmid # 81768 ; http://n2t.net/addgene:81768 ; RRID:Addgene_81768) |
| 参考文献部分: | Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC. Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. 10.1158/2159-8290.CD-16-0160 PubMed 27147599 |
买家导航
pDONR223_CHD2_WT载体基本信息
pDONR223_CHD2_WT载体图谱质粒图谱和多克隆位点信息
pDONR223_CHD2_WT载体简介
pDONR223_CHD2_WT载体序列
LOCUS pDONR223_CHD2_WT 4299 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Gateway Donor vector containing CHD2 , part of the Target
Accelerator Plasmid Collection..
ACCESSION .
VERSION .
KEYWORDS pDONR223_CHD2_WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4299)
AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG,
Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
WC
TITLE Systematic Functional Interrogation of Rare Cancer Variants
Identifies Oncogenic Alleles.
JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi:
10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
PUBMED 27147599
REFERENCE 2 (bases 1 to 4299)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4299
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 92..109
/label=L4440
/note="L4440 vector, forward primer"
terminator 268..295
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 387..473
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 522..544
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 536..553
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 537..553
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 569..668
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind complement(2178..2277)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(2294..2313)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2295..2313)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2318..2334)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2318..2334)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 2765..3556
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 3649..4237
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4138..4157
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661 agttggcacc atgatgagaa ataaggacaa aagccaagag gaggacagtt cgctacacag
721 caatgcatcg agtcactcag cctctgaaga agcttcgggt tcagactcag gcagtcagtc
781 ggaaagtgag cagggaagtg atccaggaag tggacatggc agcgagtcga acagcagctc
841 tgaatcttct gagagtcagt cggaatctga gagcgaatca gcaggttcca aatcccagcc
901 agtcctccca gaagccaaag agaagccagc ctctaagaag gaacggatag ctgatgtgaa
961 gaagatgtgg gaagaatatc ctgatgttta tggggtcagg cggtcaaacc gaagcagaca
1021 agaaccatcg cgatttaata ttaaggaaga ggcaagtagc gggtctgaga gtgggagccc
1081 aaaaagaaga ggccagaggc agctgaaaaa acaagaaaaa tggaaacagg aaccctcaga
1141 agatgaacag gaacaaggca ccagtgcaga gagtgagcca gaacaaaaaa aagtaaaagc
1201 cagaagacct gtccccagaa gaacagtgcc caaacctcgt gttaaaaagc agccgaagac
1261 tcagcgtgga aagagaaaaa agcaagattc ttctgatgag gatgatgatg atgacgaagc
1321 tcccaaaagg cagactcgtc gaagagcggc taaaaacgtt agttacaaag aagatgatga
1381 ctttgagact gactcagatg atctcattga aatgactgga gaaggagttg atgaacagca
1441 agataatagt gaaactattg aaaaggtctt agattcaaga ctgggaaaga aaggagccac
1501 tggagcatct actactgtat atgcgattga agctaatggc gaccctagtg gtgactttga
1561 cactgaaaag gatgaaggtg aaatccagta cctcatcaag tggaagggtt ggtcttacat
1621 ccacagcaca tgggagagtg aagaatcctt acagcaacag aaagtgaagg gcctaaaaaa
1681 actagagaac ttcaagaaaa aagaggacga aatcaaacaa tggttaggga aagtttctcc
1741 tgaagatgta gaatatttca attgccaaca ggagctggct tcagagttga ataaacagta
1801 tcagatagta gaaagagtaa tagctgtgaa gacaagtaaa tctacattgg gtcaaacaga
1861 ttttccagct catagtcgga agccggcacc ctcaaatgag cccgaatatc tatgtaaatg
1921 gatgggactc ccctattcag agtgtagctg ggaagatgaa gccctcattg gaaagaaatt
1981 ccagaattgc attgacagct tccacagtag gaacaactca aaaaccatcc caacaagaga
2041 atgcaaggcc ctgaagcaga gaccacgatt tgtagcttta aagaaacaac ctgcatattt
2101 aggaggggag aatctggaac ttcgagatta tcagctagaa ggtctaaact ggctagctca
2161 ttcctggtgc aagtgattgc caactttctt gtacaaagtt ggcattataa gaaagcattg
2221 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
2281 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
2341 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
2401 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
2461 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
2521 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
2581 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
2641 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
2701 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
2761 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
2821 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
2881 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
2941 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
3001 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
3061 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
3121 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
3181 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
3241 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
3301 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
3361 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
3421 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
3481 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
3541 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
3601 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
3661 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
3721 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
3781 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
3841 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
3901 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
3961 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
4021 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
4081 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
4141 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
4201 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
4261 acggttcctg gccttttgct ggccttttgc tcacatgtt
//
